Application of Rp-hplc–ed Assay to Analysis of the Blood of Parkinson’s Disease Patients

Results from application of reversed-phase HPLC with electrochemical detection to analysis of catecholamines and total antioxidant potential are presented. The assay was used to estimate their daily profiles in blood serum of patients treated with anti-Parkinson drugs. It was found that increases in the concentration of hydroxy radicals in blood serum, and of the total antioxidant potential related to the hydroxy radical, was inversely correlated with the concentration of catecholamines in the serum. This suggests that catecholamines are pro-oxidants of hydroxy radicals. The method also enabled us to study changes in the concentrations of catecholamines during their storage at room temperature. It was found that change of drug color was caused by the oxidation of benseraside.